Diagnosis of Fungal Infections (Infectious Disease and by Johan Maertens, Kieren A. Marr

By Johan Maertens, Kieren A. Marr

Interpreting a key explanation for infectious morbidity in immunosuppressed and immunocompromised sufferers, this resource spans the newest ideas to enhance and expedite the analysis, id, and therapy of fungal infections. With authoritative contributions from skilled clinicians within the box, this reference tracks advancements in imaging, susceptibility trying out, and pressure typing to wrestle those stipulations in transplant, surgical, oncology, and extensive care devices.

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12. Verweij PE, Mellado E, Melchers WJ. Multiple-triazole-resistant aspergillosis. N Engl J Med 2007; 356:1481–3. 13. Yuen KY, Woo PC, Ip MS, et al. Stage-specific manifestations of mold infections in bone marrow transplant recipients: risk factors and clinical significance of positive concentrated smears. Clin Infect Dis 1997; 25:37–42. 14. Hope WW, Walsh TJ, Denning DW. Laboratory diagnosis of invasive aspergillosis. Lancet Infect Dis 2005; 5:609–22. 15. Merz WG, Roberts GD. Algorithms for detection and identification of fungi.

The hyphae do not take in the dyes and give a negative staining, which can easily be missed, especially when the smear is examined at high magnification. wet preparation can be made to stain with the fluorescent dyes, but if such material is no longer available, the Gram-stained preparation can be used to stain with the fluorescent dye. Other stains commonly used for fungi are the India Ink which is used to detect encapsulated yeasts, such as Cryptococcus and the Giemsa stain, which is useful for detection of Histoplasma capsulatum in bone marrow and Pneumocystis jiroveci in respiratory secretions.

Sullivan DJ, Westerneng TJ, Haynes KA, Bennett DE, Coleman DC. Candida dubliniensis spp. : phenotypic and molecular characterization of a novel species associated with oral candidosis in HIV-infected individuals. Microbiology 1995; 141:1507–21. 54. Pinjon E, Moran GP, Coleman DC, Sullivan DJ. Azole susceptibility and resistance in Candida dubliniensis. Biochem Soc Trans 2005; 33:1210–4. 55. Balajee SA, Gribskov JL, Hanley E, Nickle D, Marr KA. Aspergillus lentulus spp. , a new sibling species of A.

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