Antifungal Agents: Methods And Protocols (Methods in by Erika J. Ernst

By Erika J. Ernst

A suite of state of the art molecular tools for learning antifungal resistance, for locating and comparing either new and current antifungal medications, and for realizing the host reaction and immunotherapy of such brokers. The protocols stick to the profitable tools in Molecular medication™ sequence structure, each one providing step by step laboratory directions, an advent outlining the main in the back of the strategy, lists of the mandatory apparatus and reagents, and pointers on troubleshooting and keeping off recognized pitfalls. Antifungal brokers: tools and Protocols deals clinician-scientists, microbiologists and molecular biologists the effective instruments they want this present day to appreciate and effectively improve new healing brokers for yeast, mildew, and fungal infections.

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For the construction of homozygous C. albicans mutants, however, the MPAR marker has to be recycled after insertion into the first allele of the target gene to allow its subsequent use in a second round of transformation for inactivation of the remaining wild-type allele. Because a negative selection against the presence of the MPAR marker is not available in C. albicans wild-type strains, we developed a method that allowed the specific excision of the marker from the genome of transformants with high efficiency.

Coke, A. , Burnie, J. , Lucas, G. , and Liu Yin, J. A. (1994) Invasive aspergillosis: clusters and sources? J. Med. Vet. Mycol. 32, 217–224. 18. Meunier, J. , and Grimont, P. A. (1993) Factors affecting reproducibility of random amplified polymorphic DNA fingerprinting. Res. Microbiol. 144, 373–379. CARE-2 Fingerprinting of C. albicans Isolates 27 3 CARE-2 Fingerprinting of Candida albicans Isolates Joachim Morschhäuser, Peter Staib, and Gerwald Köhler Summary The emergence of resistance to antifungal drugs in medically important fungi has become a significant problem in recent years.

Mol Microbiol 32, 547–556. 4. , and Morschhäuser, J. (2000) Targeted gene disruption in Candida albicans wild-type strains: the role of the MDR1 gene in fluconazole resistance of clinical Candida albicans isolates. Mol. Microbiol. 36, 856–865. 5. Köhler, G. , White, T. , and Agabian, N. (1997) Overexpression of a cloned IMP dehydrogenase gene of Candida albicans confers resistance to the specific inhibitor mycophenolic acid. J. Bacteriol. 179, 2331–2338. 6. , et al. (1999) Host-induced, stage-specific virulence gene activation in Candida albicans during infection.

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